Question

What formula do you use to determine the final concentration of Laemmli buffer in the sample mixes?

Answer 1

To find the final concentration of Laemmli buffer, the Henderson-Hasselbalch equation is used to calculate the mixture's components' concentrations, and then masses are calculated and mixed appropriately to create the buffer.

Step-by-step explanation:

To determine the final concentration of Laemmli buffer in the sample mixes, one can use the Henderson-Hasselbalch equation. Begin by calculating the concentrations of tris ([A]) and tris-HCl ([HA]) using the starting concentrations and the amount of acid or base added. Then, follow these steps:

1. Use the Henderson-Hasselbalch equation to find the concentrations of [A] (tris) as x and [HA] (tris-HCl) as (0.2 - x).
2. Calculate the masses needed for the buffer components.
3. Mix the calculated masses with Millipore water in a 100.00 ml volumetric flask and fill to the mark.
4. Transfer the prepared buffer to a bottle and label it appropriately with the buffer concentration, pH, your name(s), and the date.

For the ion product calculation or dilution problems, convert volumes to liters if necessary and use the initial concentrations to calculate final molarity. The concentration of ions such as Ba2+ post-mixing, but pre-reaction, can be determined by dividing the total moles of Ba2+ by the final volume after mixing.