Final answer:
The wavelength at which fluorescence of the Coomassie dye is measured can vary depending on the specific experiment and application. In general, fluorescence is measured at the emission wavelength, but the excitation wavelength is also important. The exact wavelength would depend on the experimental setup and objectives.
Step-by-step explanation:
The wavelength at which fluorescence of the Coomassie dye is measured can vary depending on the specific experiment or application. In general, fluorescence is measured at the wavelength of emitted light, which is also known as the emission wavelength. However, the excitation wavelength, which is the wavelength of light used to excite the sample, is also an important factor.
In spectrofluorometry, a technique used to measure fluorescence, the emitted light is typically measured at different wavelengths using a monochromator. The specific wavelengths used for measurement can be set by the experimenter based on the desired parameters and the properties of the fluorophore.
Therefore, the exact wavelength at which fluorescence of the Coomassie dye is measured would depend on the experimental setup and the specific objectives of the study.