Question

Bob the biochemist wants to purify a GTPase from the cell using affinity chromatography. Which molecule should he NOT attach to the column in this purification?_____

a. the GTPase's GEF
b. GTP
c. the GTPase's GAP
d. GDP
e. All of these molecules would allow for GTPase purification.

Answer 1

In affinity chromatography for GTPase purification, Bob the biochemist should NOT attach GTP to the column. Instead, he should attach the GTPase's GEF molecule to the column.

Step-by-step explanation:

In this case, Bob the biochemist should NOT attach the molecule GTP to the column in the purification process.

When using affinity chromatography to purify a GTPase from a cell, the column should be attached with a molecule that specifically binds to the GTPase. This molecule is usually the GTPase's GEF (Guanine Nucleotide Exchange Factor), which is responsible for exchanging GDP (Guanosine Diphosphate) bound to the GTPase with GTP (Guanosine Triphosphate).

The GTP molecule itself will not help in the purification process because it is the substrate that the GTPase hydrolyzes during its normal enzymatic function. Therefore, attaching GTP to the column would not allow for GTPase purification.