Question

Which proteins are necessary to maintain the separated strands during PhiX174 replication of the leading strand?

a) Cdt1, VirE
b) Rep, SSB
c) Orc1, CDK1
d) SeqA, Rep

Answer 1

During PhiX174 replication of the leading strand, the proteins Rep (helicasae) and single-strand binding proteins (SSB) are essential for maintaining the separated DNA strands.

Step-by-step explanation:

The proteins necessary to maintain the separated strands during PhiX174 replication of the leading strand are Rep and single-strand binding proteins (SSB). Rep is a helicase that unwinds the DNA at the origin of replication.

SSBs then bind to the strands, stabilizing them and preventing the re-annealing of the DNA strands during replication.