Question

What 2 individual small RNA sequences are cut out and loaded onto cas9?

Answer 1

In the CRISPR/Cas9 system, two guide RNA sequences are cut out and loaded onto Cas9. These guide RNAs direct Cas9 to the target DNA for editing.

Step-by-step explanation:

In the CRISPR/Cas9 system, two individual small RNA sequences known as guide RNAs (or gRNAs) are cut out and loaded onto Cas9. These gRNAs are responsible for guiding the Cas9 protein to the specific target DNA sequence that needs to be edited. Once the Cas9-gRNA complex binds to the target DNA, the Cas9 protein cuts both strands of the DNA, allowing for DNA repair and subsequent insertion of the desired mutations.