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Which steps during the translation of bacterial mRNA involve an interaction between complementary strands of RNA?

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Final answer:

During bacterial mRNA translation, complementary RNA interactions occur during elongation when tRNA anticodons pair with mRNA codons and during initiation when the Shine-Dalgarno sequence pairs with rRNA in the ribosome.

Step-by-step explanation:

During the translation of bacterial mRNA, the steps that involve an interaction between complementary strands of RNA are primarily during the elongation stage of protein synthesis. In this stage, the transfer RNA (tRNA) anticodon forms a complementary base-pair with the messenger RNA (mRNA) codon. As the ribosome progresses along the mRNA, translating the nucleotide sequence into a polypeptide chain, each tRNA brings a specific amino acid to the ribosome corresponding to the mRNA codon sequence. This ensures a specific and accurate translation of the mRNA into the polypeptide product.

The initiation stage also features complementary interactions, where the Shine-Dalgarno sequence on the mRNA pairs with a sequence on the small ribosomal subunit (16S rRNA in prokaryotes). This interaction sets the stage for proper alignment of the ribosome with the mRNA's start codon. Termination of translation doesn't directly involve complementary base pairing; instead, it occurs when a stop codon is reached, and a release factor binds to this codon, triggering the disassembly of the translation apparatus and release of the newly synthesized protein.

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