Final answer:
RNA polymerase II transcribes past the end of the gene and the additional nucleotides are removed during mRNA processing, meaning the stop sequence is not immediately part of the final mRNA transcript.
Step-by-step explanation:
The question asks whether the stop sequence is included during the transcription by RNA polymerase II (Pol II). In eukaryotes, transcription termination for Pol II is different from RNA polymerase I and III. RNA Pol II transcribes beyond the end of the gene before terminating, adding 1,000-2,000 extra nucleotides. These additional sequences, not part of the final mRNA that codes for protein, are subsequently removed during mRNA processing, indicating that the stop sequence itself is not immediately included in the final mRNA transcript.
RNA polymerase I and III have different mechanisms for termination that either include specific termination sequences or rely on structural formations in the RNA that lead to termination.