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Which nucleic methods provide information on microbial community aspects?

a. DNA fingerprinting and DNA sequencing
b. DNA fingerprinting, FISH, and DNA sequencing
c. DNA fingerprinting, microarray, and DNA sequencing
d. RT-PCR, microarray, and DNA sequencing

1 Answer

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Final answer:

Methods that provide information on microbial communities include DNA fingerprinting, FISH, and DNA sequencing, offering insights into species identification, spatial distribution, and function within ecosystems.

Step-by-step explanation:

The nucleic acid methods that provide information on microbial community aspects include DNA fingerprinting, Fluorescence in situ hybridization (FISH), and DNA sequencing. DNA fingerprinting is a technique based on PCR for determining unique sequences in human DNA, often used for identification in forensic science. However, in the context of microbial communities, this method can distinguish between different microbial species or strains by comparing patterns in their DNA sequences.

FISH involves the use of fluorescent probes that hybridize to specific DNA sequences within the cells. This method helps visualize the spatial distribution of microbes in environmental samples and can be used to identify which microbes are present within a community.

DNA sequencing technologies, such as the shotgun sequencing approach, read through the genetic material of microbes and provide comprehensive information about the species present and their potential functions within a community.

These methodologies, particularly when combined, give researchers a more complete picture of the makeup and dynamics of microbial ecosystems than any one technique alone could provide.

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