Question

A master mix contains a mixture of all the reagents needed for a PCR. What are the reagents?

A) DNA, primers, and nucleotides
B) Primers, nucleotides, and polymerase
C) DNA, RNA, and nucleotides
D) RNA, nucleotides, and polymerase

Answer 1

The reagents needed for a PCR are DNA template, primers, DNA polymerase, and deoxynucleotides (dNTPs).

Step-by-step explanation:

The reagents needed for a PCR (Polymerase Chain Reaction) are DNA template, primers, DNA polymerase, and deoxynucleotides (dNTPs).

DNA template: This is the target DNA sequence that is going to be amplified in the PCR reaction.

Primers: These are short pieces of DNA that are complementary to the ends of the target DNA sequence. They serve as starting points for DNA synthesis.

DNA polymerase: This enzyme synthesizes new DNA strands using the DNA template and the primers.

Deoxynucleotides (dNTPs): These are the building blocks of DNA and are needed for DNA synthesis.