Question

A tech is asked to write a procedure to measure the Evan blue concentration on a spectrophotometer. The tech is given 4 standard dilutions of Evan blue:

-Std A= 0.8 mg/dL
-Std B= 1.6 mg/dL
-Std C= 2.4 mg/dL
-Std D= 4.0 mg/dL
The first step is to:

Answer 1

To measure the Evan blue concentration on a spectrophotometer, the first step is to create a standard curve using the provided standard dilutions. The absorbance readings of these dilutions are measured and plotted on a graph to create the curve. The curve is then used to determine the concentration of an unknown Evan blue sample.

Step-by-step explanation:

The first step to measure the Evan blue concentration on a spectrophotometer is to create a standard curve using the provided standard dilutions of Evan blue. These dilutions have known concentrations (Std A = 0.8 mg/dL, Std B = 1.6 mg/dL, Std C = 2.4 mg/dL, and Std D = 4.0 mg/dL). The spectrophotometer is then set to the appropriate wavelength, and the absorbance readings of the standard dilutions are measured.

By plotting the absorbance values of the standard dilutions on a graph and connecting the points, a standard curve is created. This curve can then be used to determine the concentration of an unknown Evan blue sample by measuring its absorbance and finding the corresponding concentration on the standard curve.