Question

How to make a Lineweaver-Burk plot in Excel?

Options:
Option 1: Utilize the "Insert Scatter Plot" function
Option 2: Choose the "Line Chart" option from the toolbar
Option 3: Apply the "Surface Chart" feature
Option 4: Employ the "Bubble Chart" tool

Answer 1

To make a Lineweaver-Burk plot in Excel, utilize the "Insert Scatter Plot" function, input your enzyme kinetics data, and add a trendline to display the enzyme kinetics constants. Finally, adjust your plot's axis titles and scale as needed.

Step-by-step explanation:

To make a Lineweaver-Burk plot in Excel, you should utilize the "Insert Scatter Plot" function. Here's a step-by-step guide:

1. Enter your enzyme kinetics data into two columns, with substrate concentration (1/[S]) data in one column and corresponding velocity (1/V) data in another column.
2. Select your data and navigate to the 'Insert' tab in Excel.
3. Click on 'Scatter' and choose the first option for a plain scatter plot with markers only.
4. The scatter plot will appear on your Excel sheet, displaying your enzyme kinetics data.
5. To add a trendline (the line of best fit), right-click on any data point and choose 'Add Trendline'.
6. In the trendline options, select 'Linear' for the trendline type and choose to display the equation on the chart.
7. Adjust the axis titles and scale if necessary, to appropriately represent the Lineweaver-Burk plot.

The Lineweaver-Burk plot is used in enzyme kinetics and is a double reciprocal plot that helps determine key constants such as Km and Vmax for an enzyme-catalyzed reaction.