Final answer:
The Iroquois Confederacy established the first multi-tribal alliance against British colonial expansion.
Step-by-step explanation:
The first multi-tribal alliance against British colonial expansion was established by the Iroquois Confederacy. While the decision to remain neutral was not unanimous among the tribes, the Iroquois Confederacy declared neutrality during the American Revolutionary War. However, individual tribes proceeded to create alliances, mostly with the British.
In the South, the majority of the tribes that became involved sided with the British, except for the Catawba of North Carolina who fought on the side of the Americans. Additionally, while most Cherokee elders favored neutrality, younger tribal members rallied against the colonials and wreaked havoc on Tennessee, Kentucky, North Carolina, and Georgia.