Question

Put the three basic steps of the polymerase chain reaction (PCR) in order. Start with the earliest step at the top.

a) Denaturation, Annealing, Extension
b) Annealing, Denaturation, Extension
c) Extension, Denaturation, Annealing
d) Denaturation, Extension, Annealing

Answer 1

The three basic steps of the PCR in order are denaturation, annealing, and extension. Denaturation involves heating the DNA, annealing involves cooling the single strands and mixing them with primers, and extension involves DNA polymerase extending the strands from the primers.

Step-by-step explanation:

The three basic steps of the polymerase chain reaction (PCR) in order are: a) Denaturation, Annealing, Extension. First, denaturation involves heating DNA to break the bonds holding together the two DNA strands, yielding two single strands of DNA. Then, annealing involves cooling the single DNA strands and mixing them with short DNA segments called primers, allowing bonds to form between the DNA strands and primers. Finally, extension occurs, where DNA polymerase extends the DNA strands from the primers, creating new DNA fragments.