Question

Four cosmid clones - A, B, C, and D - were hybridized to each other in pairwise combinations. The insert size of each cosmid was also determined. The following results were obtained:

a) True
b) False

Answer 1

The question concerns advanced concepts in Biology, focusing on genetic engineering and molecular cloning using cosmids, which are significant in genetic mapping and constructing genomic libraries in molecular biology.

Step-by-step explanation:

The question relates to the subject of Biology, specifically within the field of genetic engineering and molecular cloning using cosmids as cloning vectors. Cosmids are a type of cloning vector used to clone DNA fragments up to 40 Kb in size and are instrumental in constructing genomic libraries. The question presents a scenario involving the pairwise hybridization of four cosmid clones (A, B, C, and D), which likely refers to an experiment to map genes or determine overlap among cloned DNA segments. The question also touches on concepts such as transgenic crops, recombinant DNA, PCR reactions, and the use of restriction endonucleases in cloning, which are fundamental topics in molecular biology.