Final answer:
The correct reaction during DNA replication when a deoxyribonucleotide triphosphate (dNTP) is used as a substrate is B) dNTP -> dNMP + PPi, where DNA polymerase facilitates the energy-driven formation of a phosphodiester bond.
Step-by-step explanation:
During DNA replication, the reaction that occurs on a substrate deoxyribonucleotide triphosphate (dNTP) is B) dNTP -> dNMP + PPi. This reaction involves DNA polymerase catalyzing the formation of a phosphodiester bond between the 3' OH end of the growing DNA strand and the 5' phosphate of the incoming dNTP. In this process, two of the phosphate groups (pyrophosphate, or PPi) are released from the dNTP, which provides the necessary energy for the formation of the new bond. This step is crucial for extending the DNA chain and is driven by the energy released from the cleavage of the high-energy anhydride bond in the dNTP molecule.