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Tissue Culture Stage 2 Multiplication:

a) Callus formation
b) Shoot initiation
c) Rooting
d) All of the above

1 Answer

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Final answer:

In Stage 2 Multiplication of tissue culture, 'all of the above' processes occur, including callus formation, shoot initiation, and rooting, which are all part of plantlet development. Apical meristems are critical as they are zones of rapid cell division, leading to plant growth. Micropropagation is the process enabling production of numerous plants in a laboratory.

Step-by-step explanation:

The process in tissue culture Stage 2 Multiplication that describes the proliferation of plant cells into undifferentiated mass and further development into plantlets is d) All of the above. This stage involves several key steps: callus formation, where an explant develops into an undifferentiated mass; shoot initiation, where shoot buds form from the callus; and rooting, where roots develop, preparing the plantlets for transplantation.

Apical meristems play a critical role throughout this process, as they are regions of concentrated cell division, leading to the elongation and formation of new shoots and roots. The meristematic cells are undifferentiated, allowing them to give rise to various specialized tissues.

Micropropagation is the cutting-edge process that makes it possible to rapidly produce a large number of plants from a single plant under laboratory conditions. It's essential for the propagation of rare species, economically important plants, and for producing disease-free plants.

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