Question

For the experiment on the d1s80 locus, each of the following is a step in running the electrophoresis gel after you have amplified your DNA. Place these steps in order from what you will do first to last.

a) Loading the DNA samples onto the gel
b) Running the electrophoresis apparatus
c) Staining the gel to visualize DNA bands
d) Applying an electric current to the gel

Answer 1

To analyse DNA at the d1s80 locus using gel electrophoresis, sequentially load the DNA onto the gel, apply an electric current, run the electrophoresis apparatus, and then stain the gel to visualize the DNA bands.

Step-by-step explanation:

Steps in Gel Electrophoresis

To perform gel electrophoresis of DNA at the d1s80 locus after amplification, the following steps are taken in the order presented:

1. Loading the DNA samples onto the gel: Introduce the amplified DNA into the wells of the agarose gel.
2. Applying an electric current to the gel: Start the electrophoresis by turning on the power supply to generate an electric field across the gel.
3. Running the electrophoresis apparatus: Allow the DNA to separate by size with smaller fragments moving faster towards the positive electrode.
4. Staining the gel to visualize DNA bands: After the run is completed, use a DNA-specific dye such as ethidium bromide to stain the gel and then visualize the DNA bands under UV light.

In summary, the process begins with loading the DNA onto the gel, which is followed by application of an electric current, which allows the DNA to separate during the electrophoresis run. Finally, the DNA bands are stained to make them visible for analysis.