Question

Angiotensin II is an octapeptide that is used to treat hypotension resulting from septic shock or other distributive shocks. 1. Assess the purity of Angiotensin II using Polyacrylamide Gel Electrophoresis 2. The amino acid analysis gives (Ile, Phe, Pro, Arg, Asp, His, Tyr, Val). Partial hydrolysis gives five fragments, which are sequenced by several cycles of Edman degradation as: Pro-Phe, Ile-His-Pro, Arg-Val-Tyr, Asp-Arg-Val, Val-Tyr-Ile What is the sequence?​

Answer 1

Angiotensin II can be assessed for purity using Polyacrylamide Gel Electrophoresis. The sequence of Angiotensin II based on the provided information is Pro-Phe-Ile-His-Pro-Arg-Val-Tyr-Asp-Arg-Val-Tyr-Ile.

Step-by-step explanation:

Angiotensin II is a peptide hormone used to treat hypotension resulting from septic shock or other distributive shocks. Assessing the purity of Angiotensin II can be done using Polyacrylamide Gel Electrophoresis (PAGE), a technique that separates proteins based on their size and charge. PAGE would help determine if the Angiotensin II sample is pure or if there are any impurities present. The amino acid analysis provided (Ile, Phe, Pro, Arg, Asp, His, Tyr, Val) gives insight into the composition of Angiotensin II.

As for the sequence, the partial hydrolysis of Angiotensin II yields five fragments: Pro-Phe, Ile-His-Pro, Arg-Val-Tyr, Asp-Arg-Val, and Val-Tyr-Ile. The sequenced fragments from cycles of Edman degradation indicate the order of these fragments, leading to the sequence: Pro-Phe-Ile-His-Pro-Arg-Val-Tyr-Asp-Arg-Val-Tyr-Ile.