Final answer:
Beer's Law indicates a linear relationship between absorbance and concentration, which is crucial for determining the concentrations of various compounds in biochemistry using UV-visible spectroscopy.
Step-by-step explanation:
Beer's Law states that there is a linear relationship between the concentration of a solution and its absorbance. When plotted on a graph with absorbance on the y-axis and concentration on the x-axis, the expected result is a straight line, indicating that as concentration increases, absorbance also increases in a proportional manner. This is important in fields such as biochemistry where measurements of compounds like DNA, RNA, or protein concentrations are commonly determined using UV-visible spectroscopy and based on this principle.
For example, the concentration of DNA can be estimated by measuring its absorbance at 260nm. The Beer-Lambert law for this case is represented as Absorbance (at 260nm) = ε*c*l, where ε is the absorption coefficient, c is the concentration, and l is the path length. Similarly, protein concentrations can be determined through assays that produce a color change correlating to protein quantity, with the relationship between protein concentration and absorbance being depicted on a standard curve.