Question

In your lab notebook, prepare data tables with the calculated volumes needed for each of the five stocks (ZY, 50x M, 1000x metals, 1 M MgSO4, 50 mg/ml kanamycin) needed to prepare autoinduction base medium, and each of the 50x carbon sources.

a) Design data tables for the experiment.

b) Identify the key variables in the experiment.

c) Analyze the impact of each stock on the autoinduction base medium.

d) Determine the optimal concentrations for each stock.

Answer 1

To design data tables for the experiment, include columns for each stock and rows for each assay. The key variables in the experiment are the volumes and concentrations of the stocks. Analyzing the impact of each stock involves determining how they affect protein expression. Optimal concentrations can be determined through preliminary experiments.

Step-by-step explanation:

To design data tables for the experiment, you need to create a table with the calculated volumes for each of the five stocks, including ZY, 50x M, 1000x metals, 1 M MgSO4, and 50 mg/ml kanamycin, needed to prepare autoinduction base medium and each of the 50x carbon sources. In this table, you should include columns for each stock and rows for each assay.

The key variables in the experiment are the volumes of each stock added to the autoinduction base medium and the concentrations of the stocks. Analyzing the impact of each stock on the medium involves determining how they affect the growth and expression of the desired protein. The optimal concentrations for each stock can be determined by conducting preliminary experiments and optimizing the conditions for protein expression.