Final answer:
The Jamestown colonists did not endure harsh winters as a significant hardship, but they did experience lack of reliable drinking water, starvation, and diseases.
Step-by-step explanation:
The final answer to which hardship was not endured by the colonists in Jamestown is a) harsh winters. The hardships experienced by the Jamestown settlers included lack of dependable drinking water, starvation, and malnutrition and disease. The settlers suffered immensely due to the sweltering summer heat, the damp chill of the cold winters, and the lack of adequate resources. Internal political disputes and conflicts with the native Powhatan tribe exacerbated their difficulties.
Moreover, a focus on searching for non-existent mineral wealth, rather than growing food, added to their distress. The winter of 1609-1610, known as 'the starving time,' nearly led to the colony's failure until the arrival of a supply ship from England provided relief.