Final answer:
Electrophoresis is the correct answer as it does not require PCR. It's a method used to separate molecules like DNA or proteins by size and charge without amplifying them, unlike other techniques listed.
Step-by-step explanation:
The question asks which analysis tool does not include Polymerase Chain Reaction (PCR). Among the options provided, the correct answer is D: electrophoresis. This is because electrophoresis is a technique used to separate DNA, RNA, or protein molecules based on their size and charge by applying an electric current to them.
Unlike STR profiling, SNP analysis, and Y-STR analysis, which all require PCR for amplifying specific regions of DNA for analysis, electrophoresis can be used for analyzing DNA fragments that have been previously amplified or cut with restriction enzymes, as in RFLP (restriction fragment length polymorphism) analysis.