Question

You get an OD of 0.7 after dilution. Calculate the OD600 of the original culture.

Answer 1

The OD₆₀₀ of the original culture is 1.4286.

Step-by-step explanation:

The optical density (OD) is a measure of the concentration of cells in a culture, and it is commonly measured at a wavelength of 600 nm (OD₆₀₀). When a culture is diluted, the relationship between the original and diluted OD can be expressed using the formula:

OD original = OD diluted x Dilution factor

In this case, the dilution factor is given as 0.7. Rearranging the formula to solve for the original OD:

OD original = OD diluted x Dilution factor

Substituting the given values:

OD original = 0.7x0.7

OD original = 1.4286

Therefore, the OD₆₀₀ of the original culture is 1.4286.

In biological research, maintaining accurate cell concentrations is crucial for experimental reproducibility. Optical density provides a quick and convenient method for estimating cell density without the need for time-consuming cell counts. When a culture is diluted, the OD is proportionally reduced, and the original OD can be calculated by multiplying the diluted OD by the dilution factor. This calculation assumes a linear relationship between cell concentration and OD, making it a useful tool in microbiology and cell biology research.

Answer 2

The OD₆₀₀ of the original culture is 1.4286.

Step-by-step explanation:

The optical density (OD) is a measure of the concentration of cells in a culture, and it is commonly measured at a wavelength of 600 nm (OD₆₀₀). When a culture is diluted, the relationship between the original and diluted OD can be expressed using the formula:

`\[ \text{OD}_\text{original} = \text{OD}_\text{diluted} * \text{Dilution factor} \]`

In this case, the dilution factor is given as 0.7. Rearranging the formula to solve for the original OD:

`\[ \text{OD}_\text{original} = \text{OD}_\text{diluted} * \text{Dilution factor} \]`

Substituting the given values:

`\[ \text{OD}_\text{original} = 0.7 * 0.7 \]`

`\[ \text{OD}_\text{original} = 1.4286 \]`

Therefore, the OD₆₀₀ of the original culture is 1.4286.

In biological research, maintaining accurate cell concentrations is crucial for experimental reproducibility. Optical density provides a quick and convenient method for estimating cell density without the need for time-consuming cell counts. When a culture is diluted, the OD is proportionally reduced, and the original OD can be calculated by multiplying the diluted OD by the dilution factor. This calculation assumes a linear relationship between cell concentration and OD, making it a useful tool in microbiology and cell biology research.