Final answer:
Gram-positive bacteria, with their thick peptidoglycan cell walls, are susceptible to antibiotics that target cell wall synthesis, such as penicillin.
Step-by-step explanation:
When performing Gram staining, the type of bacteria that are susceptible to antibiotics affecting peptidoglycan cell walls are Gram-positive bacteria. These bacteria have thick cell walls consisting predominantly of peptidoglycan, which retains the purple Gram stain.
Antibiotics like penicillin target the synthesis of peptidoglycan, effectively combating Gram-positive bacterial infections. In contrast, Gram-negative bacteria have a thinner peptidoglycan layer and an additional outer membrane that makes them more resistant to such antibiotics.