Question

in the experiment, you will first make a standard curve by plotting absorbance of nadh vs. alcohol concentration by starting with the same nad concentration but varying the alcohol concentration. what a slope do you expect to get? group of answer choices positive slope no answer text provided. i don not know. negative slope

Answer 1

In this experiment, a negative slope is expected when plotting the absorbance of NADH against the concentration of alcohol. There is a negative relationship between NADH absorbance and alcohol concentration.

Step-by-step explanation:

In this experiment, you will create a standard curve by plotting the absorbance of NADH against the concentration of alcohol. By starting with the same NADH concentration but varying the alcohol concentration, you will obtain a plot with a negative slope.

The negative slope indicates a negative relationship between the absorbance of NADH and the concentration of alcohol. As the concentration of alcohol increases, the absorbance of NADH decreases. This is because the alcohol competes with NADH for absorption of light at 340 nm.

The negative slope in the standard curve helps establish a relationship that can be used to determine the concentration of alcohol in unknown samples based on their absorbance value.