The primer with a higher GC content will have a higher melting point.
The melting point of a primer is influenced by its GC content. The more GC pairs in a primer, the higher its melting point.
If two primers have the same G and A content but are arranged differently, we would expect the primer with a higher GC content to have a higher melting point. This is because GC pairs have three hydrogen bonds, while AT pairs have only two.
Therefore, the primer with a higher GC content will have a higher melting point.
The complete question is- you order two primers from a company that synthesizes oligonucleotides. the primers have the exact same g and a content, but arranged differently. which one would you expect to have the higher melting point?