Rho-independent transcription termination requires the formation of a stem-loop structure in the mRNA and a uracil-rich sequence at the 3' end of the mRNA, leading to the dissociation of the RNA polymerase from the DNA.
The rho-independent mechanism of transcription termination does not rely on the rho protein. Instead, it requires the formation of a specific sequence of events in the DNA template:
The formation of a stem-loop structure at the 3' end of the nascent mRNA which occurs when the polymerase transcribes a region rich in C-G nucleotides.
Following the stem-loop, a uracil-rich sequence at the 3' end of the mRNA is transcribed. This weakens the interaction between the RNA and DNA, making it easier for the RNA polymerase to dissociate, effectively terminating transcription.
These sequences lead to the stalling of the RNA polymerase and the destabilization of the DNA-RNA complex, allowing for the newly formed mRNA chain to be released.
The appropriate question is:
what does the rho-independent mechanism of transcription termination require? multiple select question.
a) a helicase to separate the dna-rna complex
b) the presence of a stop codon in the mrna
c) the formation of a stem-loop structure
d) a uracil-rich sequence located at the 3' end of the mrna