Different liquid chromatography detectors respond to different types of analytes: UV detectors to substances absorbing ultraviolet light, refractive index detectors to any changes in refractive index, evaporative light scattering detectors to non-volatile analytes, charged aerosol detectors to ionizable particles, electrochemical detectors to substances able to oxidize or reduce, fluorescence detectors to fluorescent compounds, nitrogen chemiluminescence detectors to nitrogen-containing compounds, and conductivity detectors to ionic analytes.
The question you've asked concerns the types of analytes to which different liquid chromatography detectors respond. Here are the detectors and their corresponding analytes:
a. Ultraviolet (UV): This detector responds to analytes that absorb ultraviolet light, which typically includes compounds with conjugated double bonds or aromatic rings.
b. Refractive index: A refractive index detector responds to any analyte that causes a change in the refractive index of the mobile phase.
c. Evaporative light scattering: Detects all non-volatile analytes as they affect the scattering of light when evaporated.
d. Charged aerosol: Targets analytes that can be ionized into aerosol particles in a charged state.
e. Electrochemical: Sensitive to analytes that can undergo oxidation or reduction.
f. Fluorescence: Responds to compounds that can fluoresce or can be made to fluoresce after derivatization.
g. Nitrogen chemiluminescence: Specifically sensitive to compounds containing nitrogen that produce light upon undergoing a chemiluminescent reaction.
h. Conductivity: Measures the ability of ions present in the analyte to conduct electricity.
These detectors are integral to liquid chromatography (LC) and are selected based on the specific properties of the analytes being studied.
The complete question is- o which kinds of analytes do these liquid chromatography detectors respond?
(a) ultraviolet
(b) refractive index
(c) evaporative light scattering
(d) charged aerosol
(e) electrochemical
(f) fluorescence
(g) nitrogen chemiluminescence
(h) conductivity