Question

The array CGH utilizes two genomic DNA samples which are labeled differently. Which combination of DNA samples and labeling are we using today? a. Patient DNA labeled with green fluorophore, control DNA labeled with red fluorophore b. Patient DNA labeled with red fluorophore, control DNA labeled with green fluorophore c. Patient DNA labeled with biotin, control DNA labeled with FITC d. Patient DNA labeled with Cy3, control DNA labeled with Cy5 e. Fetal DNA with Cy3 and reference DNA with Cy5

Answer 1

The correct combination of DNA samples and labeling for array CGH today, based on the standard practice, is to label patient DNA with Cy3 and control DNA with Cy5, as noted in option d.

Step-by-step explanation:

Array comparative genomic hybridization (CGH) involves the comparison of two genomic DNA samples by labeling them with different fluorescent dyes and hybridizing them to a microarray chip. In practice, the combination of DNA samples and labeling can vary, but one common method is to label patient DNA with a red fluorophore and control DNA with a green fluorophore. Specifically in the context of the answer choices provided, option d, which involves labeling patient DNA with Cy3 (a red fluorophore) and control DNA with Cy5 (a far-red fluorophore), is consistent with the standard practice of differentially labeling genomic DNA for array CGH analysis. Once the labeled DNA is combined and hybridized to the array, the hybridization patterns can be interpreted based on the color emitted at each spot, providing crucial information about genomic differences.

Learn more about Array CGH Labeling