Final answer:
The Fifth Amendment right that was violated in Chambers v. Florida (1940) was the defendants' right to remain silent.
Step-by-step explanation:
In Chambers v. Florida (1940), the Fifth Amendment right that was violated was the defendants' right to remain silent. In this case, the defendants were coerced into making self-incriminating statements by police officers without being properly informed of their right to stay silent and avoid self-incrimination.
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