Final answer:
DNA ligase is the enzyme responsible for connecting the Okazaki fragments on the lagging strand during DNA replication.
Step-by-step explanation:
The enzyme responsible for connecting the Okazaki fragments of the lagging strand in DNA replication is DNA ligase. Okazaki fragments are short segments of DNA synthesized on the lagging strand during DNA replication. They are created in discrete pieces because the lagging strand is synthesized in the opposite direction of the replication fork.
Learn more about DNA replication and enzymes