Final answer:
Lack of water and low turgor pressure cause a plant's guard cells to close stomata.
Step-by-step explanation:
Stomata are small openings in the epidermis of leaves and stems that allow for gas exchange in plants. The guard cells surround the stomata and control their opening and closing. Two factors that can cause a plant's guard cells to close its stomata are lack of water and low turgor pressure. When there is a lack of water, the guard cells lose turgor pressure and become flaccid, causing the stomata to close and reduce water loss through transpiration.
Learn more about Plant physiology