Question

Increase salt in DNA will lead to

a. Increase Tm
b.
Decrease Tm
c. No change
d. Both are correct

Answer 1

Increasing salt in DNA decreases the melting temperature (Tm)

Step-by-step explanation:

Increasing salt in DNA will lead to decrease Tm. The melting temperature (Tm) is the temperature at which half of the DNA strands in a solution are denatured. Higher salt concentrations in the solution interfere with the hydrogen bonding between the DNA strands, making it easier for the strands to separate and therefore lowering the Tm.

Learn more about effect of salt on DNA melting temperature