Question

What is the concentration when the absorbance is 0.510? a. 3 µg/10µl b. 7.5 µg/µl c. 0.66 µg/µl d. 16 µg/10µl​

Answer 1

To determine the concentration when the absorbance is 0.510, we need additional information such as the molar absorptivity.

Step-by-step explanation:

The concentration of a sample can be determined using the equation for absorbance:

A = εbc

where A is the absorbance, ε (epsilon) is the molar absorptivity, b is the path length, and c is the concentration. Rearranging the equation to solve for concentration, we have:

c = A / (εb)

Plugging in the values for absorbance (0.510) and path length (10 µl), and given that the molar absorptivity (ε) is not provided, we cannot determine the concentration without further information.

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