Question

Identify one intracellular change that could be measured during T cell activation. Draw a simple graph, labeling the y-axis as the intracellular change and the x- axis with a group of T cells alone, vs. T cells with dendritic cells (APC)

Answer 1

CD69 expression can be measured as an intracellular change during T cell activation.

A graph comparing T cells alone and T cells with dendritic cells can be used to measure this change.

What is the intracellular change?

One intracellular change that could be measured during T cell activation is the expression of CD69 on the surface of T cells. CD69 is an early activation marker and its expression increases upon T cell activation.

A simple graph could be created to compare the level of CD69 expression on T cells alone and T cells with dendritic cells (APC) by measuring the mean fluorescence intensity (MFI) on the y-axis and labeling the x-axis with the two groups.