Question

what percent of the mrfp1 containing puc19-207 plasmids would express a functional mrfp1 protein if a biology 207 student successfully inserted the mrfp1 gene into puc19-207 using a single digest? assume the mrfp1 gene is promoterless. (1 mark)

Answer 1

If the mRFP1 gene was successfully inserted into the pUC19-207 plasmid using a single digest, all of the resulting plasmids should be capable of expressing the functional mRFP1 protein.

Step-by-step explanation:

If the mRFP1 gene was successfully inserted into the pUC19-207 plasmid using a single digest, the percent of the resulting plasmids that would express a functional mRFP1 protein depends on the efficiency of the insertion. Assuming the insertion is successful, all of the plasmids containing the mRFP1 gene should be capable of expressing the functional protein. However, it's important to note that the efficiency of insertion can vary and not all plasmids may contain the inserted gene.

Learn more about expression of mRFP1 protein