Final answer:
The best replacement for 'Open' is 'Opening', as it functions as the subject of the sentence
Step-by-step explanation:
The best replacement for the underlined word in the sentence 'Open his chemistry book, Trevor knew he had a long night of studying ahead of him.' is Opening. The word 'Opening' is a gerund that acts as the subject of the verb 'knew'. A gerund is a verb form ending in -ing that functions as a noun. In this sentence, 'Opening' functions as the subject of the sentence, indicating what action Trevor was taking at that moment.
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