- Tissue culture is a technique used in biology and biotechnology to grow and maintain living cells or tissues outside their natural environment.
- The process involves selecting suitable source materials, sterilizing them, preparing explants, and creating a nutrient-rich culture medium.
- The explants are placed onto the culture medium and incubated under controlled conditions to promote cell growth and development.
- Subculturing is done periodically to maintain optimal conditions for growth and prevent overcrowding.
- Tissue culture finds applications in plant propagation, disease-free plant production, genetic modification, conservation of endangered species, medical and veterinary research, drug discovery, vaccine production, artificial tissue development, human medical research, drug testing, regenerative medicine, in vitro fertilization (IVF), and biotechnology and pharmaceutical industries.
- It is a valuable tool for studying cellular processes, advancing medical research, and enabling large-scale production of biological products.