Answer:
The main difference between the traditional alkaline lysis method and the kit method of isolating DNA is the way DNA is collected in the last few steps. In the traditional alkaline lysis method, DNA is collected by ethanol precipitation, where the DNA is pelleted by centrifugation and washed with ethanol to remove any contaminants. On the other hand, in the kit method, DNA is collected by binding the DNA to a silica column, washing away contaminants, and then eluting the purified DNA from the column. The kit method is more efficient and less time-consuming than the traditional method and yields high-quality DNA suitable for downstream applications.