DNA replication occurs in both prokaryotes and eukaryotes, but there are some differences between the two processes. Both require a template DNA strand, enzymes, and occur in a 5' to 3' direction. However, prokaryotes have a smaller genome size, replication occurs in a single location, and the process is faster. Eukaryotes have a larger genome size, replication occurs at multiple sites, and three different polymerases are involved. Eukaryotes also have proofreading mechanisms to correct errors, while prokaryotes do not.