Answer:
Step-by-step explanation:
Immersion oil is used in light microscopy to increase the resolution of the image by reducing the amount of light that is scattered as it passes through the specimen. To apply immersion oil correctly, the following steps should be followed:
Focus the microscope on the specimen of interest using the 40x or higher objective lens.
Rotate the nosepiece to the 100x (or higher) objective lens and move the lens into position over the specimen.
Apply a small drop of immersion oil to the center of the specimen.
Use the fine adjustment knob to move the objective lens closer to the specimen until the lens is almost touching the oil drop.
Slowly turn the focusing knob to move the lens slightly away from the specimen until the image comes into focus.
Do not move the slide or the lens while viewing the specimen.
When finished, use lens paper to wipe off any excess oil from the objective lens.
It is important to use immersion oil correctly and to clean the objective lens thoroughly after use to prevent any residue from interfering with the performance of the lens. Using immersion oil in the correct sequence will help to produce a clearer, higher resolution image in light microscopy.