Amidst the challenging environment, the development of Jamestown was threatened by both the settlers and the Powhatans. The settlers, who were unprepared for the difficult conditions and relied heavily on the Powhatans for sustenance and resources, were vulnerable to a range of adversities, including sickness and hostile attacks. Conversely, the Powhatans viewed the settlers as a threat to their way of life and land and were determined to protect their interests. The inevitable conflict between the two groups resulted in a complex and intricate relationship that had a significant impact on Jamestown's development.