Question

Describe the TWO ways in which CRISPR works in gene editing

Answer 1

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) works in gene editing through two main mechanisms:

CRISPR-Cas9: This mechanism involves the use of the Cas9 endonuclease enzyme and a guide RNA molecule (gRNA) to find and cut the specific DNA target. The broken DNA is then repaired by the cell's natural processes, resulting in specific mutations or changes in the genetic code.

CRISPR-Cpf1: Similar to CRISPR-Cas9, this mechanism also involves the use of a nuclease enzyme (Cpf1) and a gRNA to target and cut DNA. However, it has some distinct differences from CRISPR-Cas9, such as its ability to target different sites within the genome and create a more stable cut in DNA.

Step-by-step explanation:

Both of these CRISPR-based mechanisms have the potential to be used for a variety of applications, including the treatment of genetic diseases and the development of new treatments for various medical conditions.


ALLEN

Answer 2

Crispr / cas 9 edits genes by precisely cutting dna and then letting natural dna repair processes to take over. The system consists of two parts: the cas 9 enzyme and a guide rna. Rapidly translating a revolutionary technology into transformative therapies.