Answer: B. A natural disaster occurred that separated squirrels.
Step-by-step explanation: Based on the given options, the event that is most likely to have caused the population of squirrels to diverge into two different species over a long period of time is a natural disaster that occurred and separated the squirrels.
A natural disaster, such as an earthquake or flood can physically separate populations of squirrels by creating geographical barriers. These barriers can prevent gene flow between the two groups, leading to genetic isolation and divergence over time.
When populations of squirrels are separated by these barriers, it limits their ability to interact and breed with one another. This lack of gene flow between the two groups can lead to genetic isolation. Over time, the isolated populations may accumulate different genetic variations and undergo genetic changes through mechanisms such as genetic drift and natural selection. These changes can eventually result in the formation of new species.