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In dna fingerprinting what do restriction enzymes do

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Answer:

breakdown of DNA into small pieces

Step-by-step explanation:

  • We use restriction enzymes to digest the DNA and break it into smaller fragments .

Steps in DNA fingerprinting:-

  • Firstly we isolate the DNA .
  • Breakdown of DNA using restriction endonucleases .
  • Seperation of fragments by Agarose Gel electrophoresis
  • Transferring this digested DNA into nitrocellulose membrane ( or any other synthetic membrane)
  • Hybridisation using VNTR probe
  • Detection of fragments by autoradiography.
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