D. A bat would be least affected by sound frequencies of 25,000 to 100,000 cycles per second. Bats use ultrasonic frequencies in their echolocation calls to navigate and locate prey, they have specialized ears and auditory systems that allow them to hear and process these high frequency sounds. While other animals like human, cheetah, porpoise and house cat would not be able to hear these ultrasonic frequencies and would not be affected by them.