Answer:
see below
Step-by-step explanation:
The LUC gene, which codes for the luciferase enzyme that generates bioluminescence, is frequently used as a reporter gene in molecular biology investigations. The LUC gene has to be translated into a working luciferase enzyme in order for it to become active.
The first step in the expression of a gene is transcription, which occurs when the RNA polymerase enzyme copies a specific DNA sequence into a corresponding RNA molecule. The RNA molecule is subsequently moved from the nucleus to the cytoplasm, where ribosomes carry out the process of translating it into a protein.
The regulatory components, like as promoters, enhancers, and silencers, must be present for the LUC gene to be transcribed. These factors regulate the LUC gene's accessibility to RNA polymerase as well as the rate of transcription. The RNA molecule produced by the LUC gene is translated by the ribosomes into the luciferase enzyme.
Therefore, for the LUC gene to become active, the following steps must happen:
1- The LUC gene must be present and accessible for the RNA polymerase to transcribe it.
2- The RNA polymerase must transcribe the LUC gene into an RNA molecule.
3- The RNA molecule must be transported out of the nucleus to the cytoplasm.
4- The ribosomes must translate the RNA into a functional luciferase enzyme.
It's worth mentioning that the activity of LUC gene also depends on the presence of the necessary substrate and cofactor for the enzyme to produce light.