142k views
3 votes
Haemotoxylin and eosin is a common staining technique used in microscopy. Which of the following are reasons why cells need to be stained prior to microscopy? [3 marks]

- Kill cells

- No cellular components are visible without staining

- Highlight metabolic processes

- Make all organelles homogeneous

- Highlight different cellular structures

- Differentiate between living and dead cells

1 Answer

3 votes

STAINING

The reasons why cells need to be stained prior to microscopy are:

  • No cellular components are visible without staining

  • Highlight different cellular structures

  • Differentiate between living and dead cells

Staining is a technique used in microscopy to make cells and other biological tissues visible under a microscope. Without staining, many cellular components are not visible because they are too small or transparent to be seen without some form of contrast. By applying a stain, such as haemotoxylin and eosin, to the tissue, it is possible to highlight specific structures or components within the cells, making them more visible under the microscope.

Staining can also be used to differentiate between different types of cells or tissues, or to highlight specific structures or processes within the cells. For example, different stains may be used to highlight different organelles or to visualize metabolic processes.

kill cells, is not a reason why cells need to be stained prior to microscopy.

highlight metabolic processes, is not an accurate description of staining.

make all organelles homogeneous, is not a reason why cells need to be stained prior to microscopy.

Hope This Helps You!

User Dimitris
by
6.9k points