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4. Which of the following was John Rolfe's chief contribution to Virginia's economy? A. His innovations in cultivating tobacco B. His innovations in rice cultivation C. His alliance with the Powhatan through his marriage to Pocahontas D. His invention of a better way to grow short-staple cotton

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The best option from the list would be "A. His innovations in cultivating tobacco," since tobacco quickly became the most lucrative crop in Virginia, thanks to its high output. 
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