26.3k views
0 votes
Which of the following steps is  not 

critical in the creation of Bt corn? a.Cut out a piece of DNA from a DNA molecule. b.Splice a piece of DNA into DNA from another organism. c.Use a restriction enzyme to form sticky ends in DNA. d.Read the DNA sequence of the piece of DNA to be cut and spliced.    

2 Answers

5 votes

Final answer:

Reading the entire DNA sequence of the piece to be cut and spliced is not critical in creating Bt corn; rather, using restriction enzymes and DNA ligase to splice and stitch DNA are the vital steps.

Step-by-step explanation:

The creation of Bt corn involves several critical steps utilizing restriction enzymes and DNA ligase. Restriction enzymes, such as BamHI or HaeIII, perform a critical function by splicing DNA at specific points, which may result in sticky ends or blunt ends, to allow for the insertion of new DNA fragments. DNA ligase then performs the vital role of stitching together the new foreign gene to the existing piece of DNA, finalizing the recombinant DNA sequence.

However, one step mentioned that is not critical to the creation of Bt corn involves reading the entire DNA sequence of the piece to be cut and spliced. While understanding the sequence can be important in various contexts, the specificity of restriction enzymes to their recognition sites and the action of DNA ligase do not require full sequencing of DNA fragments for the successful splicing and insertion part of the process.

User Matt Bryant
by
9.2k points
4 votes
a. Cut out a piece of DNA from a DNA molecule
How does molecular genetics add to our understanding of genes is by simply
studying them. Molecular genetics is scientific study of composition of genes. This field is a subcategory of biology and heredity which is responsible for unearthing and delving the anatomy and physiology of the genes. This field helps us to understand and find evidence in the compounds, substances and elements found and synthesized in our genes and what contains in our nucleic acides –RNA and DNA –framework of our chromosomes. And also the origin and history of how genes came to be. These are codes and like-signals that makes us who we are in a macro-perspective.


User M Rostami
by
8.9k points