Final answer:
Reading the entire DNA sequence of the piece to be cut and spliced is not critical in creating Bt corn; rather, using restriction enzymes and DNA ligase to splice and stitch DNA are the vital steps.
Step-by-step explanation:
The creation of Bt corn involves several critical steps utilizing restriction enzymes and DNA ligase. Restriction enzymes, such as BamHI or HaeIII, perform a critical function by splicing DNA at specific points, which may result in sticky ends or blunt ends, to allow for the insertion of new DNA fragments. DNA ligase then performs the vital role of stitching together the new foreign gene to the existing piece of DNA, finalizing the recombinant DNA sequence.
However, one step mentioned that is not critical to the creation of Bt corn involves reading the entire DNA sequence of the piece to be cut and spliced. While understanding the sequence can be important in various contexts, the specificity of restriction enzymes to their recognition sites and the action of DNA ligase do not require full sequencing of DNA fragments for the successful splicing and insertion part of the process.